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pgl3 human oct4 pe sv40 luc  (Addgene inc)


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    Addgene inc pgl3 human oct4 pe sv40 luc
    Enrichment of CSCs derived from endometrial carcinoma cells. A Representative images of spheres formed in serum-free medium on days 3, 6, 9, and 12. B , C Western blot analysis of stemness markers (ALDH1A1, c-Myc, Nanog, and <t>Oct4)</t> in parental cells (PCs) cultured in serum-supplemented medium and CSCs maintained in serum-free medium for 12 days. Different letters (a, b) indicate significant differences ( P < 0.05) between groups. All cytological experiments were independently repeated at least three times
    Pgl3 Human Oct4 Pe Sv40 Luc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgl3 human oct4 pe sv40 luc/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    pgl3 human oct4 pe sv40 luc - by Bioz Stars, 2026-05
    93/100 stars

    Images

    1) Product Images from "Quercetin suppresses endometrial cancer stem cells via ERα-mediated inhibition of STAT3 signaling"

    Article Title: Quercetin suppresses endometrial cancer stem cells via ERα-mediated inhibition of STAT3 signaling

    Journal: Discover Oncology

    doi: 10.1007/s12672-025-03863-8

    Enrichment of CSCs derived from endometrial carcinoma cells. A Representative images of spheres formed in serum-free medium on days 3, 6, 9, and 12. B , C Western blot analysis of stemness markers (ALDH1A1, c-Myc, Nanog, and Oct4) in parental cells (PCs) cultured in serum-supplemented medium and CSCs maintained in serum-free medium for 12 days. Different letters (a, b) indicate significant differences ( P < 0.05) between groups. All cytological experiments were independently repeated at least three times
    Figure Legend Snippet: Enrichment of CSCs derived from endometrial carcinoma cells. A Representative images of spheres formed in serum-free medium on days 3, 6, 9, and 12. B , C Western blot analysis of stemness markers (ALDH1A1, c-Myc, Nanog, and Oct4) in parental cells (PCs) cultured in serum-supplemented medium and CSCs maintained in serum-free medium for 12 days. Different letters (a, b) indicate significant differences ( P < 0.05) between groups. All cytological experiments were independently repeated at least three times

    Techniques Used: Derivative Assay, Western Blot, Cell Culture

    The effects of Quercetin treatment on sphere formation and maintenance of stemness in CSCs. A 1 × 10 4 cells were treated with 25 or 50 µmol/L Quercetin for 12 days, and spheres were imaged. B 100 spheres with diameter > 50 μm were treated with 50 µmol/L Quercetin for 72 h, and then imaged. C 1 × 10 5 cells were treated with 25 or 50 µmol/L Quercetin for 12 days, and total protein was extracted for western blot analysis of stemness markers (ALDH1A1, c-Myc, Nanog, and Oct4). Different letters (a, b, c) indicate significant differences ( P < 0.05) between groups. All cytological experiments were independently repeated at least three times
    Figure Legend Snippet: The effects of Quercetin treatment on sphere formation and maintenance of stemness in CSCs. A 1 × 10 4 cells were treated with 25 or 50 µmol/L Quercetin for 12 days, and spheres were imaged. B 100 spheres with diameter > 50 μm were treated with 50 µmol/L Quercetin for 72 h, and then imaged. C 1 × 10 5 cells were treated with 25 or 50 µmol/L Quercetin for 12 days, and total protein was extracted for western blot analysis of stemness markers (ALDH1A1, c-Myc, Nanog, and Oct4). Different letters (a, b, c) indicate significant differences ( P < 0.05) between groups. All cytological experiments were independently repeated at least three times

    Techniques Used: Western Blot

    Quercetin inhibits STAT3’s transcriptional activity in the presence of ERα. A Luciferase reporter assay was used to evaluate the inhibitory effect of Quercetin on STAT3-mediated Oct4 promoter activity. Different letters (a, b) indicate significant differences ( P < 0.05) between groups. B mRNA expression of Oct4, Nanog, Twist, and Snai1 was measured by qPCR after Quercetin treatment. Different letters (a, b, c) indicate significant differences ( P < 0.05) between groups. The effects of Quercetin on sphere formation ( C ) and invasion ( D ) were assessed in the presence or absence of ERα. All cytological experiments were independently repeated at least three times
    Figure Legend Snippet: Quercetin inhibits STAT3’s transcriptional activity in the presence of ERα. A Luciferase reporter assay was used to evaluate the inhibitory effect of Quercetin on STAT3-mediated Oct4 promoter activity. Different letters (a, b) indicate significant differences ( P < 0.05) between groups. B mRNA expression of Oct4, Nanog, Twist, and Snai1 was measured by qPCR after Quercetin treatment. Different letters (a, b, c) indicate significant differences ( P < 0.05) between groups. The effects of Quercetin on sphere formation ( C ) and invasion ( D ) were assessed in the presence or absence of ERα. All cytological experiments were independently repeated at least three times

    Techniques Used: Activity Assay, Luciferase, Reporter Assay, Expressing



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    Enrichment of CSCs derived from endometrial carcinoma cells. A Representative images of spheres formed in serum-free medium on days 3, 6, 9, and 12. B , C Western blot analysis of stemness markers (ALDH1A1, c-Myc, Nanog, and <t>Oct4)</t> in parental cells (PCs) cultured in serum-supplemented medium and CSCs maintained in serum-free medium for 12 days. Different letters (a, b) indicate significant differences ( P < 0.05) between groups. All cytological experiments were independently repeated at least three times
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    Enrichment of CSCs derived from endometrial carcinoma cells. A Representative images of spheres formed in serum-free medium on days 3, 6, 9, and 12. B , C Western blot analysis of stemness markers (ALDH1A1, c-Myc, Nanog, and <t>Oct4)</t> in parental cells (PCs) cultured in serum-supplemented medium and CSCs maintained in serum-free medium for 12 days. Different letters (a, b) indicate significant differences ( P < 0.05) between groups. All cytological experiments were independently repeated at least three times
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    Image Search Results


    Enrichment of CSCs derived from endometrial carcinoma cells. A Representative images of spheres formed in serum-free medium on days 3, 6, 9, and 12. B , C Western blot analysis of stemness markers (ALDH1A1, c-Myc, Nanog, and Oct4) in parental cells (PCs) cultured in serum-supplemented medium and CSCs maintained in serum-free medium for 12 days. Different letters (a, b) indicate significant differences ( P < 0.05) between groups. All cytological experiments were independently repeated at least three times

    Journal: Discover Oncology

    Article Title: Quercetin suppresses endometrial cancer stem cells via ERα-mediated inhibition of STAT3 signaling

    doi: 10.1007/s12672-025-03863-8

    Figure Lengend Snippet: Enrichment of CSCs derived from endometrial carcinoma cells. A Representative images of spheres formed in serum-free medium on days 3, 6, 9, and 12. B , C Western blot analysis of stemness markers (ALDH1A1, c-Myc, Nanog, and Oct4) in parental cells (PCs) cultured in serum-supplemented medium and CSCs maintained in serum-free medium for 12 days. Different letters (a, b) indicate significant differences ( P < 0.05) between groups. All cytological experiments were independently repeated at least three times

    Article Snippet: pGL3-human Oct4 PE-SV40-luc was purchase from Addgene; the vector pGL3 bought from Pomega was used as a control.

    Techniques: Derivative Assay, Western Blot, Cell Culture

    The effects of Quercetin treatment on sphere formation and maintenance of stemness in CSCs. A 1 × 10 4 cells were treated with 25 or 50 µmol/L Quercetin for 12 days, and spheres were imaged. B 100 spheres with diameter > 50 μm were treated with 50 µmol/L Quercetin for 72 h, and then imaged. C 1 × 10 5 cells were treated with 25 or 50 µmol/L Quercetin for 12 days, and total protein was extracted for western blot analysis of stemness markers (ALDH1A1, c-Myc, Nanog, and Oct4). Different letters (a, b, c) indicate significant differences ( P < 0.05) between groups. All cytological experiments were independently repeated at least three times

    Journal: Discover Oncology

    Article Title: Quercetin suppresses endometrial cancer stem cells via ERα-mediated inhibition of STAT3 signaling

    doi: 10.1007/s12672-025-03863-8

    Figure Lengend Snippet: The effects of Quercetin treatment on sphere formation and maintenance of stemness in CSCs. A 1 × 10 4 cells were treated with 25 or 50 µmol/L Quercetin for 12 days, and spheres were imaged. B 100 spheres with diameter > 50 μm were treated with 50 µmol/L Quercetin for 72 h, and then imaged. C 1 × 10 5 cells were treated with 25 or 50 µmol/L Quercetin for 12 days, and total protein was extracted for western blot analysis of stemness markers (ALDH1A1, c-Myc, Nanog, and Oct4). Different letters (a, b, c) indicate significant differences ( P < 0.05) between groups. All cytological experiments were independently repeated at least three times

    Article Snippet: pGL3-human Oct4 PE-SV40-luc was purchase from Addgene; the vector pGL3 bought from Pomega was used as a control.

    Techniques: Western Blot

    Quercetin inhibits STAT3’s transcriptional activity in the presence of ERα. A Luciferase reporter assay was used to evaluate the inhibitory effect of Quercetin on STAT3-mediated Oct4 promoter activity. Different letters (a, b) indicate significant differences ( P < 0.05) between groups. B mRNA expression of Oct4, Nanog, Twist, and Snai1 was measured by qPCR after Quercetin treatment. Different letters (a, b, c) indicate significant differences ( P < 0.05) between groups. The effects of Quercetin on sphere formation ( C ) and invasion ( D ) were assessed in the presence or absence of ERα. All cytological experiments were independently repeated at least three times

    Journal: Discover Oncology

    Article Title: Quercetin suppresses endometrial cancer stem cells via ERα-mediated inhibition of STAT3 signaling

    doi: 10.1007/s12672-025-03863-8

    Figure Lengend Snippet: Quercetin inhibits STAT3’s transcriptional activity in the presence of ERα. A Luciferase reporter assay was used to evaluate the inhibitory effect of Quercetin on STAT3-mediated Oct4 promoter activity. Different letters (a, b) indicate significant differences ( P < 0.05) between groups. B mRNA expression of Oct4, Nanog, Twist, and Snai1 was measured by qPCR after Quercetin treatment. Different letters (a, b, c) indicate significant differences ( P < 0.05) between groups. The effects of Quercetin on sphere formation ( C ) and invasion ( D ) were assessed in the presence or absence of ERα. All cytological experiments were independently repeated at least three times

    Article Snippet: pGL3-human Oct4 PE-SV40-luc was purchase from Addgene; the vector pGL3 bought from Pomega was used as a control.

    Techniques: Activity Assay, Luciferase, Reporter Assay, Expressing